About Seebio
Pioneer of Core Raw Materials for Functional Food Ingredients and Healthcare Supplements in China.
Glutathione reductase (GR) is a crucial enzyme in the human body's oxygen reduction system. As a key player in the glutathione redox cycle, it catalyzes the conversion of oxidized glutathione (GSSG) into reduced glutathione (GSH), playing a vital role in maintaining the activity and reducing properties of sulfhydryl proteins, preserving cell integrity, and preventing oxidative degradation of hemoglobin.
Measuring serum glutathione reductase activity can aid in diagnosing liver-related diseases, including hepatitis, cirrhosis, obstructive jaundice, and liver tumors.
Detection Principle of Glutathione Reductase (GR)
Glutathione reductase catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH) using NADP+H as a cofactor. During this process, NADP+H is oxidized to NADP++. NADP+H exhibits a characteristic absorption peak at 340 nm, whereas NADP++ does not absorb at this wavelength. By measuring the rate of absorbance decrease at 340 nm, we can determine the rate of NADP+H dehydrogenation, thereby calculating the activity of glutathione reductase (GR).
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NADPH ＋ H+ ＋ GSSG
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Glutathione Reductase (GR)
--------------------------------->
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NADP+ ＋ 2GSH
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Glutathione Reductase (GR) Detection Kit: Raw Materials and Accessories
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Seebio Item
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Product
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CAS
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Level
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DCL0001A
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Bovine serum albumin（BSA）
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9048-46-8
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Biotechnology Grade
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DCL0001B
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Bovine serum albumin(None protease,BSA）
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9048-46-8
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Biotechnology Grade
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AAS0020A
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Oxidized glutathione, GSSG
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27025-41-8
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≥99.8%
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EYN0147B
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β-NADPH-4Na
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ECI0077D
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oxidized coenzyme I, β-NAD
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53-84-9
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BR,98%
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EBY0025G
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D-Lactate dehydrogenase
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9028-36-8
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GradeⅡ; 400 U/mg-solid
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DCE1104B
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L-Lactate Dehydrogenase
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9001-60-9
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EEI0121A
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Glutathione Reductase (calibrator) (GR）
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9001-48-3
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EEI0121B
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Glutathione Reductase (Quality Control) (GR）
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9001-48-3
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ACH0010A
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Disodium ethylenediaminetetraacetate dihydrate (EDTA-2Na-2H2O）
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6381-92-6
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Biochemika Grade
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ACH0007A
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Ethylenediaminetetraacetic acid （EDTA）
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60-00-4
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≥99%
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ACC0013A
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4-(2-Hydroxyethyl)-1-piperazineeth anesulfonic acid（HEPES）
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7365-45-9
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≥99%
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ACC0016A
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2-(N-Morpholine)ethanesulfonic acid monohydrate （MES）
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145224-94-8
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>99%
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ACC0185A
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2-(N-Morpholino)ethanesulfonic acid sodium salt （MES-Na）
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71119-23-8
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99%
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ACC0030B
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Tris(hydroxymethyl)aminomethane （Tris base）
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77-86-1
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Ultra Pure Grade
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ACC0029A
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Tris(hydroxymethyl)aminomethane hydrochloride （Tris-HCl）
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1185-53-1
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High Purity Grade
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AHD0046B
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Potassium dihydrogen phosphate
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7778-77-0
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AR Grade
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AHD0031D
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Sodium dihydngen phoshate anhydrous
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7558-80-7
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AR
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AHD0031A
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Sodium phosphate monobasic dihydrate
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13472-35-0
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AR
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AHD0045D
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Sodium phosphate dibasic anhydrous
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7558-79-4
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AR
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AHD0017A
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Sodium phosphate dibasic dihydrate
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10028-24-7
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98%
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AHD0023A
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Sodium phosphate dibasic dodecahydrate
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10039-32-4
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AR Grade
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AHD0022A
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Sodium chloride
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7647-14-5
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GR
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AHD0120B
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Potassium chloride
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7447-40-7
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99%
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ACN0037B
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KroVin 300 Preservative
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BR
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