About Seebio
Pioneer of Core Raw Materials for Functional Food Ingredients and Healthcare Supplements in China.
Tag antibodies, also referred to as antigenic epitopes or antigenic determinants, possess the remarkable ability to specifically recognize distinct epitopes on fusion proteins created through recombinant technology. These epitopes serve as unique markers, determining the specificity of the antigen, and specifically bind to their corresponding antibodies.
As biotechnology advances, researchers frequently employ DNA recombinant techniques to construct fusion proteins incorporating target genes and epitope tags. These tags then allow for the identification and purification of these proteins through the use of specific tag antibodies, fulfilling various research requirements. Additionally, recombinant hybrids containing polypeptide fusion monomers (affinity tags) are widely utilized to aid in the purification of target proteins.
In essence, tag antibodies are indispensable tools for exploring gene protein expression, signal transduction, and gene function. To cater to the diverse needs of our customers, Seebio Biotech has independently developed a comprehensive range of over 10 types of antigen tag antibodies, encompassing HA tag antibodies, Myc tag antibodies, DYKDDDDK tag antibodies, GFP tag antibodies, and more. These tag antibodies undergo stringent quality control measures and are thoroughly validated for various applications, including ELISA, Western blot, flow cytometry, immunoprecipitation, and immunoassay fluorescence.
The utilization of tag antibodies allows for the detection and purification of tag sequences present on diverse commercial expression vectors. This process enables the analysis of the expression content and functionality of the target protein. The underlying principle involves an antigen-antibody reaction, where these tag antibodies specifically bind to their corresponding tag fusion proteins with high precision.
Introducing the various label types
Utilizing molecular biology techniques, epitope tags can be seamlessly fused to either the N- or C-terminus of the target protein. Among the commonly used epitope tags are His, HA, Myc, and GST. Notably, these epitope tags typically refrain from altering the biological activity or intracellular localization of the target protein. The advent of epitope tags has greatly facilitated both scientific research and industrial production, as commercial tag antibodies are capable of specifically recognizing the tag attached to the protein, thereby facilitating subsequent identification and analysis of the target protein.
Flag Antibody - Anti-Flag Tag Antibody
The employment of fusion tags, including Flag, GST, and others, offers a convenient means to streamline protein purification, regulate the spatial positioning of protein immobilization, and facilitate detection. They also aid in visualizing biological occurrences within the body, boosting the yield of recombinant proteins, and enhancing their solubility and stability. Commonly utilized tags encompass myc, HA, Flag, His, GST, among others. The Flag tag system incorporates a brief, hydrophilic eight-amino acid peptide (DYKDDDDK) that is fused to the target protein.
His Antibodies - Anti-His Tag Antibodies
Fusion tags can be categorized into two groups based on their relative molecular weight: large protein molecules and small polypeptide fragments. These tags serve to simplify the purification process of proteins, regulate the spatial orientation of protein immobilization, facilitate detection, visualize biological events in vivo, enhance the production of recombinant proteins, and improve their solubility and stability.
GST Antibody - Anti-GST Tag Antibody
With the discovery of an increasing number of novel genes, gene fusion protein expression systems have gained widespread application due to their remarkable advantages in the research of newly discovered proteins. Among them, the GST tag system stands out for its high protein expression yield, convenient purification of expression products, and its facilitation in the preparation of GST antibodies. The GST fusion protein is soluble in aqueous solution, enabling its extraction from bacterial lysate and subsequent purification via affinity chromatography under non-denaturing conditions. Furthermore, GST fusion proteins can be cleaved by site-specific proteases, effectively removing the GST protein. Additionally, the fusion protein serves as a robust immunogen, facilitating the preparation of antibodies against novel proteins. Precisely because of these advantages, commercialized GST fusion protein expression systems and GST tag antibody systems remain widely utilized today.
GFP Antibody - Anti-GFP Tag Antibody
Commonly used tags include GFP, HA, Flag, His, GST, among others. GFP, short for Green Fluorescent Protein, was initially discovered by Osamu Shimomura and colleagues in 1962 in the jellyfish species Aequorea victoria. The protein encoded by its gene emits a distinct green fluorescence when excited by light in the blue wavelength range.
Myc Antibody - Anti-Myc Tag Antibody
With the rapid advancement of proteomics, the utilization of recombinant proteins has significantly surged in recent years. These recombinant hybrids often incorporate affinity tags like GST, Myc, His, and others, which aid in the purification of target proteins. This approach has gained widespread acceptance. The employment of fusion proteins to facilitate the purification and detection of recombinant proteins is now widely recognized. In 1985, mouse anti-c-myc tag antibodies were developed and have since been employed as immunochemical reagents in cell biology and protein engineering. The Myc tag (sequence: EQKLISEEDL) has proven effective in applications such as WB hybridization technology, immunoprecipitation (IP), and flow cytometry. Consequently, it can be effectively used to detect the expression of recombinant proteins in bacteria, yeast, insect cells, and mammalian cells.
HA Antibodies - Anti-HA Tag Antibodies
The integration of fusion tags, including HA, His, and others, simplifies the protein purification process, controls the spatial orientation of protein fixation, and facilitates detection. These tags enable the visualization of biological events within the body, enhance the yield of recombinant proteins, and improve their solubility and stability. Commonly utilized tags encompass myc, HA, Flag, His, GST, among others. The HA tag system employs a short peptide derived from the influenza hemagglutinin epitope (YPYDVPDYA), which is fused to the target protein.
Advantages of Seebio Label Products
Multiple verifications for reliability and accuracy.
High specificity, ensuring precise targeting and minimal nonspecific binding.
Wide range of reactive species, allowing for compatibility with various experimental systems.
Good dilution ratio, enabling cost-effective use without compromising performance.
Product List
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Item number
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Product
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Specification
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Type
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Source
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Application platform
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Recommended use
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Mouse anti-BSA
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mg
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mAb
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Mouse
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ELISA, CLIA, GICA.
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Detection
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|
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Mouse anti-Avidin
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mg
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mAb
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Mouse
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ELISA, CLIA, GICA.
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Detection
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|
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Mouse anti-FITC
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mg
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mAb
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Mouse
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ELISA, CLIA, GICA.
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Detection
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|
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Mouse anti-GST Tag
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mg
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mAb
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Mouse
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ELISA, CLIA, GICA.
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Detection
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Mouse anti-His Tag
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mg
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mAb
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Mouse
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ELISA, CLIA, GICA.
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Detection
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|
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Mouse anti-MBP Tag
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mg
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mAb
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Mouse
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ELISA, CLIA, GICA.
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Detection
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DYKDDDDK (FLAG® epitope tag) antibody, mouse monoclonal antibody
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100mL
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mAb
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Mouse
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ICC/IF IF
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Identify N-terminal and C-terminal Flag tags in fusion proteins
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|
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HA tag antibody, mouse monoclonal antibody
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100mL
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mAb
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Mouse
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ELISA WB FCM IF IP ICC/IF
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Recognition of N-terminal and C-terminal HA tags in fusion proteins。
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|
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GFP tag antibody, rabbit monoclonal antibody
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100mL
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Ab
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Rabbit
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WB ELISA IP
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Recognition of N-terminal and C-terminal GFP tags in fusion proteins。
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GFP tag antibody, rabbit polyclonal antibody
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100mL
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PcAb
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Rabbit
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ELISA
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Recognition of N-terminal and C-terminal GFP tags in fusion proteins。
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EGFP tag antibody, rabbit polyclonal antibody
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100mL
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PcAb
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Rabbit
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ELISA
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|
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EGFP tag antibody, rabbit polyclonal antibody, antigen affinity purification
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100mL
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PcAb
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Rabbit
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ELISA
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