About Seebio
Pioneer of Core Raw Materials for Functional Food Ingredients and Healthcare Supplements in China.
Cytokines are low molecular weight soluble proteins produced by various cells in response to immunogens, mitogens, or other stimuli. They play a crucial role in regulating innate immunity, adaptive immunity, hematopoiesis, cell growth, APSC pluripotent cells, and the repair of damaged tissue. As a comprehensive service provider in the life sciences field, the core team at Seebio brings years of experience in biopharmaceutical R&D and production. Seebio is dedicated to the research, development, production, and sales of products in the life sciences realm, offering a diverse range of recombinant cytokine series products and gene products.
For inquiries about CRISPR-related enzyme products for editing, please contact us at service@seebio.cn or call us at +86 21 58183719, or reach us on Wechat at +86 158 0195 7578.
Features of Seebio Recombinant Cytokines
GMP Level Production Management Specifications
CHO-K1 Production Platform (Complete Post-Translational Processing, Activity Similar to Natural Protein)
Serum-Free, Animal-Derived Grade
High Purity
High Biological Activity
Protein Content Verified by Testing
Low Endotoxin Levels
CHO-K1 Production Platform (Complete Post-Translational Processing, Activity Similar to Natural Protein)
Serum-Free, Animal-Derived Grade
High Purity
High Biological Activity
Protein Content Verified by Testing
Low Endotoxin Levels
Reminder: Endotoxin is a product of the cell wall of Gram-negative bacteria. When bacteria die, autolyze, or adhere to other cells, they exhibit toxicity. The primary chemical component of endotoxin is the lipid A component of lipopolysaccharide. Endotoxin binds to receptors on the cell membrane, triggering an immune response and exerting a potent toxic effect on cells. Our products utilize various methods to effectively remove endotoxins.
Classification of Cytokines
Interleukin (IL-s): Cytokines produced by white blood cells acting between white blood cells.
Interferon (IFN): Early-discovered cytokines with the ability to interfere with viral infection and replication (α, β, and g types).
Tumor Necrosis Factor Superfamily (Tumor Necrosis Factor, TNF): Discovered in 1975, inducing hemorrhage and necrosis in tumors.
Colony-Stimulating Factor (CSF): Cytokines stimulating the proliferation and differentiation of pluripotent hematopoietic stem cells and various hematopoietic progenitor cells.
Biological Functions of Cytokines
Cytokines play a pivotal role in mediating interactions between cells and exhibit diverse biological functions:
Mediation of Natural Immunity: Participate in anti-tumor and anti-infection responses.
Regulation of T and B Cell Functions: Modulate activation, growth, and differentiation, influencing cellular and humoral immunity.
Stimulation of Hematopoiesis: Induce the growth and differentiation of bone marrow progenitor cells into various mature blood cells.
Involvement in Inflammation, Infection, and Endotoxemia: Contribute to the body's response in these situations.
Role in Hypersensitivity and Autoimmune Diseases: Influence the development of immune-related disorders.
Induction of Cell Proliferation and Differentiation: Activate corresponding receptors (CKR) leading to cell proliferation, differentiation, or the secretion of specific proteins.
Cytokines exhibit high efficiency and can exert their effects in micromolar or even picomolar amounts. The impact of a single cytokine on immunity depends on factors such as local cytokine concentrations, receptor expression patterns, and the integration of signaling pathways in immune response cells. Cytokines act as molecular messengers facilitating communication among immune system cells, coordinating responses to target antigens, and playing regulatory and effector roles in various diseases. Consequently, cytokines and their receptors find applications in immunotherapy.
Cytokines and Immunotherapy
In immunotherapy, cytokines directly stimulate immune effector cells and stromal cells at the tumor site, enhancing cytotoxicity. Extensive research on animal tumor models has revealed the broad anti-tumor activities of cytokines, leading to the approval of numerous cytokine drugs by the FDA. As immunomodulators, cytokines can activate or suppress immunotherapy, encompassing recombinant, synthetic, and natural preparations. Cytokines also play a crucial role in cellular immunotherapy, including NK, CART, and DC-CIK therapies.
Monoclonal Antibodies in Immunotherapy
Recombinant Anti-PD-1 Fully Human Monoclonal Antibody (GMP Grade)
PD-1 (Programmed Cell Death Protein 1) is an essential immunosuppressive transmembrane protein expressed on T cells. Antibodies targeting PD-1 have been recognized as broad-spectrum anti-tumor drugs and potential tools for balanced cell therapy. PD-1, present on the surface of activated T cells, B cells, and myeloid cells, interacts with ligands PD-L1 and PD-L2. In the tumor microenvironment, cancer cells expressing these ligands can evade immune system detection by inhibiting T cell activation. Therapies blocking PD-1 connections to its ligands, through antibodies or genetic manipulation, enhance tumor eradication and improve cancer immunity.
Chemokine: Recruiting monocytes, neutrophils, lymphocytes, etc., to specific lymphoid organs, tissues, and infection sites.
Growth Factor (GF): Cytokines stimulating cell growth.
Mouse Anti-Human CD3 Monoclonal Antibody & Anti-Human CD28 Monoclonal Antibody
The CD3 molecule serves as a crucial marker for T cells, composed of five polypeptide chains: γ, δ, ε, ζ, and eta. Their cytoplasmic regions all contain Immunoreceptor Tyrosine-based Activation Motifs (ITAM), facilitating the transmission of T Cell Receptor (TCR) signals to activate and proliferate T cells.
CD28 is a transmembrane protein with a relative molecular mass of 44,000, belonging to the immunoglobulin family. Its costimulatory signal induction can trigger the differentiation, proliferation, and interleukin-2 (IL-2) secretion of T cells. Monoclonal antibodies targeting CD28 effectively provide costimulatory signals, promoting T cell proliferation. They have demonstrated significant application value in tumor immunity treatment, autoimmune diseases, and immune intervention against transplant rejection.
Mononuclear cells isolated from peripheral blood, bone marrow, or cord blood can be cultured in the presence of CD3 and CD28 monoclonal antibodies, along with various cytokines, to obtain Cytokine-induced Killer (CIK) cells with tumor cell-killing activity.
Currently, both domestic and international studies utilize a combination of anti-CD3/CD28 antibodies to activate T lymphocytes in vitro. The synergistic effect of anti-CD28 antibodies significantly enhances the stimulatory activity of anti-CD3 antibodies. Therefore, in clinical applications, anti-CD28 antibodies are employed in conjunction with anti-CD3 antibodies for the in vitro activation of T lymphocytes.
Seebio Cytokine Series Products
Seebio offers a range of recombinant cytokines and CRISPR-related products for gene editing, catering to the needs of immunology researchers. These products are consistently available in stock, and packaging options can be customized to meet specific requirements.
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Product name
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Packaging
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Expression system
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Recombinant anti-PD-1 fully human monoclonal antibody
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1mg
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CHO-K1
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Recombinant Her-2 fully human monoclonal antibody
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2mg/10mg
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CHO-K1
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Anti-human CD3 monoclonal antibody (mouse source）
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1mg
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CHO-K1
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Anti-human CD28 monoclonal antibody (mouse source）
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1mg
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CHO-K1
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Recombinant human Activin A
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50ug/1mg
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CHO-K1
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Recombinant human brain-derived neurotrophic factor (rhBDNF）
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50ug/1mg
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CHO-K1
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Recombinant human basic fibroblast growth factor (rhbFGF）
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50ug/1mg
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CHO-K1
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Recombinant human BMP-2 (rhBMP-2）
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50ug/1mg
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CHO-K1
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Recombinant human BMP-4 (rhBMP-4）
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50ug/1mg
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CHO-K1
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Recombinant human BMP-7 (rhBMP-7）
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50ug/1mg
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CHO-K1
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Recombinant human CD40L (rhCD40L)
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50ug/1mg
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CHO-K1
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Recombinant human Dkk1 (rhDkk1)
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50ug/1mg
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CHO-K1
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Recombinant human epidermal growth factor (rhEGF）
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1mg
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CHO-K1
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Recombinant human erythropoietin (rhEPO）
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1mg
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CHO-K1
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Recombinant human Flt3L (rhFLt3L)
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50ug/1mg
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CHO-K1
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Recombinant human keratinocyte growth factor-2 (rhKGF-2/FGF-10)
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50ug/1mg
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CHO-K1
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Recombinant human fibroblast growth factor-4 (rhFGF4)
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50ug/1mg
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CHO-K1
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Recombinant human fibroblast growth factor-9 (rhFGF9)
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50ug/1mg
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CHO-K1
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Recombinant human fibronectin (rhFN）
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1mg
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Yeast
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Recombinant human glial cell-derived neurotrophic factor (rhGDNF)
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50ug/1mg
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CHO-K1
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Recombinant human hepatocyte growth factor (rhHGF)
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50ug/1mg
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CHO-K1
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Recombinant human interferon-γ (rhIFN-γ)
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100ug/1mg
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CHO-K1
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Recombinant human interleukin-1a (rhIL-1a)
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50ug/1mg
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CHO-K1
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Recombinant human interleukin-2 (rhIL-2)
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1000KU/1mg
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CHO-K1
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Recombinant human interleukin-3 (rhIL-3)
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50ug/1mg
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CHO-K1
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Recombinant human interleukin-4 (rhIL-4)
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50ug/1mg
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CHO-K1
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Recombinant human interleukin-6 (rhIL-6)
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50ug/1mg
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CHO-K1
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Recombinant human interleukin-7 (rhIL-7）
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50ug/1mg
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CHO-K1
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Recombinant human interleukin-12 (rhIL- 12)
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50ug/1mg
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CHO-K1
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Recombinant human interleukin-15 (rhIL-15）
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50ug/1mg
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CHO-K1
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Recombinant human interleukin-18 (rhIL-18）
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50ug/1mg
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CHO-K1
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Recombinant human interleukin-21 (rhIL-21）
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50ug/1mg
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CHO-K1
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Recombinant human leukemia inhibitory factor (rhLIF）
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50ug/1mg
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CHO-K1
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Recombinant human long-chain insulin-like growth factor-1 (rhLR3-IGF-1)
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1mg
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CHO-K1
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Recombinant human Noggin (rhNoggin)
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50ug/1mg
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CHO-K1
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Recombinant human NRG-1 (rhNRG-1)
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50ug/1mg
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CHO-K1
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Recombinant human platelet-derived growth factor (rhPDGF-BB）
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1mg
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CHO-K1
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Recombinant human R-spondin (rhR-spondin)
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50ug/1mg
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CHO-K1
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Recombinant human stem cell factor (rhSCF)
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50ug/1mg
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CHO-K1
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Recombinant human sonic hedgehog (rhSHH)
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50ug/1mg
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CHO-K1
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Recombinant human tumor necrosis factor (rhTNF-a）
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50ug/1mg
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CHO-K1
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Recombinant human transforming growth factor (rhTGFβ1）
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50ug/1mg
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CHO-K1
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Recombinant human vascular endothelial growth factor (rhVEGF)
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50ug/1mg
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CHO-K1
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Recombinant human Wnt-3α (rhWnt-3α)
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50ug/1mg
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CHO-K1
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Recombinant human insulin (rhInsulin)
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1g/10g
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Yeast
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Bovine Transferrin)
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1g/10g
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Yeast
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Recombinant human interferon α2b (rhIFN-α2b）
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1mg
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Yeast
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Recombinant human granulocyte colony-stimulating factor (rhG-CSF)
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1mg
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Yeast
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Recombinant human granulocyte macrophage stimulating factor (rhGM-CSF)
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100ug/1mg
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Yeast
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