In the blood analysis process of a hematology analyzer, the use of various supporting reagents is crucial, and each step influences the attainment of accurate results. For instance, substandard physical and chemical indicators in the diluent can lead to elevated platelet (PLT) background, poor white blood cell (WBC) classification, and hematocrit (HCT) drift. Hemolytic agents with incomplete or excessive hemolysis can distort white blood cell count results, while cleaning agents impact the instrument's blank value and blanking rate. Apart from the formulation ratio, the selection of raw materials is a pivotal factor influencing these effects.

 

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Common Classifications of Hematology Reagents

 

Hematology analyzers typically employ three key reagents: diluent, hemolytic agent, and cleaning solution.

 

1 Diluent

Blood cell diluent is commonly an isotonic solution with acid-base buffering effects, utilizing buffers such as ADA, TRIS, HEPES, imidazole, etc. It maintains appropriate ionic strength and conductivity. Diluent is vital for blood cell analyzers in clinical testing, playing a crucial role in blood cell classification or counting. Additionally, it collaborates with the cleaning solution before analysis to reduce instrument background values and prevent interference from previous test samples. Diluent also helps preserve the original shape of white blood cells and platelets, ensuring accurate classification by the instrument.

 

2 Hemolytic Agent

A hemolytic reagent is essential for counting white blood cells and determining hemoglobin (HGB) content. It dissolves red blood cells, releasing hemoglobin, which combines with components in the hemolytic agent to form derivatives. Colorimetry at a specific wavelength, usually 530 to 550nm, measures the absorbance change, directly proportional to hemoglobin content. Hemolytic agents induce differences among white blood cell types, enabling the instrument to classify and count various groups.

 

3 Cleaning Fluid

Cleaning fluid is designed to cleanse the instrument pipeline of blood and remove proteins, minimizing cross-contamination and ensuring result reliability and accuracy. It creates a humid environment, swiftly eliminating air bubbles from the entire flow system. Ingredients often include high-quality amphoteric surfactants, animal hydrolytic proteases, preservatives, sodium formate, sodium chloride, and buffers such as ADA, TRIS, HEPES, and imidazole. The cleaning agent is indispensable for the hematology analyzer, a crucial factor in obtaining accurate test results.

 

4 Other Reagents

Additional reagents used in blood cell analysis include anticoagulants. The International Committee for Standardization (ICSH) recommends EDTA as the preferred anticoagulant for blood samples in counting and screening blood cells.

 

Accurate test results depend on the correct use of these analysis reagents.

 

 

Product List

 

Item number	Product name	CAS No.
Buffer
ACC0013A	4-(2-Hydroxyethyl)-1-piperazineeth anesulfonic acid	7365-45-9
ACC0030B	Tris(hydroxymethyl) aminomethane,tris base	77-86-1
ACC0036A	Tris(hydroxymethyl) aminomethane,tris base	64431-96-5
AHD0006A	1,3-Bis[tris(hydroxymethyl)methylamino]propane	1303-96-4
AHB0001A	Boric acid	10043-35-3
AHD0031A	Sodium phosphate monobasic dihydrate	13472-35-0
ACP0005A	Glycine	56-40-6
AJL0030D	Maleic acid	110-16-7
Quaternary ammonium salt
ACH0168A	n-Octyl trimethyl ammonium chloride	10108-86-8
ACH0170A	n-Decyl trimethyl ammonium chloride	10108-87-9
ACH0020A	Dodecyl trimethyl ammonium chloride	112-00-5
ACH0019A	Dodecyl trimethyl ammonium bromide	1119-94-4
ACH0021A	Tetradecyl trimethyl ammonium chloride
ACH0022A	Tetradecyl trimethyl ammonium bromide	1119-97-7
ACH0024A	Cetyl trimethyl ammonium chloride	112-02-7
ACH0023A	Cetyl trimethyl ammonium bromide	57-09-0
Surfactant
ACH0017B	Sodium dodecyl sulfate	151-21-3
ACH0026A	Surfactant S14
ACH0028A	Surfactant S20
ACH0003B	Surfactant S21
ACH0107A	Surfactant S23
ACH0010A	Ethylenediaminetetraacetic acid disodium salt dihydrate	6381-92-6
ACH0008A	Dipotassium salt dihydrate	25102-12-9
ACC0015A	Imidazole	288-32-4
ACH0113A	Emulsifier AEO-9
ACH0376A	Emulsifier OP-10
Preservative
ACN0046A	Krovin 500
ACN0047B	Krovin 600
ACN0043B	Krovin 950