Taq DNA Polymerase, commonly known as Taq enzyme, is a widely used DNA polymerase in molecular biology. Isolated from the thermophilic bacterium Thermus aquaticus, this enzyme is renowned for its high thermal stability, allowing it to maintain activity under high-temperature conditions. After 2 hours at 70°C, Taq DNA Polymerase retains more than 90% of its original activity, and at 95°C, it maintains approximately 60% of its activity. This property makes it an excellent choice for applications such as molecular cloning and polymerase chain reaction (PCR) to amplify specific DNA fragments in vitro. The enzyme’s resilience during the denaturation step of PCR, which typically reaches around 94°C, means it can seamlessly proceed to subsequent cycles without the need for additional enzyme additions, positioning it as the optimal enzyme for specialized PCR reactions.

 

 

Seebio® sTaq DNA Polymerase is a novel variant of Taq DNA Polymerase developed leveraging advancements in PCR technology and modern genetic engineering techniques. This product boasts a modified Taq DNA Polymerase with enhanced reaction efficiency and guaranteed safety. Its superior fidelity and resistance to impurity interference ensure amplification efficiency and detection sensitivity.

 

With 5’→3’ polymerase activity and lacking 5’→3’ exonuclease activity, but possessing 3’→5’ exonuclease activity, the PCR amplification product features a 3’-dA overhang, facilitating cloning into T vectors and direct utilization for TA cloning.

 

For more product details, please contact us: service@seebio.cn or Phone: +86 21 58183719 or Wechat: +86 158 0195 7578

 

Characteristics:

User-friendly - Pre-mixed format for simplified operation, requiring only the addition of template DNA and primers to perform PCR.

High fidelity - boasting 50 times the fidelity of conventional Taq polymerase, with the mismatch rate significantly reduced to 10^-6, minimizing the chance of errors.

High specificity - Offers exceptional hot start performance to control non-specific amplification and enzyme activity loss at low temperatures, ensuring the specificity and stability of PCR amplification.

High synthesis speed - Faster synthesis speeds lead to shorter PCR reaction times, conserving amplification time and maintaining higher enzyme activity.

High salt tolerance - Greater tolerance allows for a wider adaptability of PCR amplification and suitability for a broader range of samples.

 

Quality Control:

 

 

 

Scope of Application   

 

Suitable for PCR amplification of conventional DNA fragments, high-throughput PCR, DNA labeling, primer extension, sequence determination, and blunt-end A addition, among others. Useful for blood diagnostic PCR, bacterial liquid PCR, and the development of diagnostic PCR products.

 

The product undergoes rigorous quality control processes to ensure its reliability and performance in various applications.

 

Product Components  

 

 

 

Storage conditions and precautions    

-20℃ Storage ≥24months

It is stable when stored at 4°C for short periods of time. Repeated freezing and thawing will reduce enzyme activity; mix gently before use and centrifuge quickly to collect.

 

This product is only intended for use in scientific research and not for human uses.

 

 

Molecular diagnostic related enzyme products   

 

Seebio Biotech can provide a variety of molecular diagnostic enzyme products, all of which can be customized in large packaging.